The W.E.B. Du Bois Boyhood Homesite (or W.E.B. Du Bois Homesite) is a National Historic Landmark in Great Barrington, Massachusetts, commemorating an important location in the life of African American intellectual and civil rights activist W.E.B. Du Bois (1868–1963).
The site contains foundational remnants of the home of Du Bois' grandfather, where Du Bois lived for the first five years of his life. Du Bois was given the house in 1928, and planned to renovate it, but was unable to do so. He sold it in 1954 and the house was torn down later that decade.
The site is located on South Egremont Road (state routes 23 and 41), west of the junction with Route 71.